Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis typing of Acinetobacter baumannii in China.
نویسندگان
چکیده
A panel of seven variable-number tandem-repeat (VNTR) markers was selected for Acinetobacter baumannii typing analysis (MLVA-7). Compared with pulsed-field gel electrophoresis (PFGE), MLVA-7 provided greater discrimination. We modified the criteria for MLVA complex assignments proposed previously, and a remarkable congruence between MLVA-7- and PFGE-based strain clustering was observed.
منابع مشابه
Multiple - Locus Variable - Number Tandem - Repeat Analysis of 1 Acinetobacter baumannii in China : comparison with PFGE typing
Acinetobacter baumannii in China: comparison with PFGE typing. 2 Yuan Hu, Boqing Li, Dazhi Jin, Zhigang Cui, Xiaoxia Tao, Binghua Zhang, 3 Jianzhong Zhang 4 5 1 State Key Laboratory for Infectious Disease Prevention and Control, National 6 Institute for Communicable Disease Control and Prevention, Chinese Center for 7 Disease Control and Prevention, 2 Binzhou Medical College, Yantai, china, 3 8...
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In search of an effective DNA typing technique for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new target sequence was evaluated. Using known genomic sequences of Acinetobacter baumannii strains AR 319754 and ATCC 17978, we developed single-locus variable-number direct-repeat analysis using polymerase chain reaction-restriction fragment le...
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OBJECTIVE To develop a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) assay for Acinetobacter pittii typing. METHODS Polymorphic VNTRs were searched by Tandem Repeats Finder. The distribution and polymorphism of each VNTR locus were analyzed in all the A. pittii genomes deposited in the NCBI genome database by BLAST and were evaluated with a collection of 20 well-characte...
متن کاملMultiple-locus variable-number tandem repeat analysis for typing of Staphylococcus epidermidis.
We applied a high-resolution PCR-based typing method, multiple-locus variable-number tandem repeat analysis (MLVA), for discrimination of 30 multidrug-resistant clinical isolates of Staphylococcus epidermidis. The results of MLVA were congruent with results obtained by pulsed-field gel electrophoresis (PFGE). MLVA generated discrete character data, and its discriminatory capacity was comparable...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 51 4 شماره
صفحات -
تاریخ انتشار 2013